- It is a physical method of separation.
- the components separated are distributed between 2 phases
- "mobile phase" and a stationary phase
- It involves a mixture dissolving in a "mobile phase" though a stationary phase.
- It is a method of separating and analyzing mixtures of chemicals.
- The separation process, especially of closely related compounds, by allowing a solution or mixture to seep through an absorbent (such as clay, gel, or paper).
- so each compound becomes absorbed into a separate, often colored, layer.
- Different components travel at different rates.
Sheet Chromatography
Paper Chromatography (PC):
- Is a technique that involves placing a small dot or line of sample solution onto a strip of chromatography paper.
- The paper is placed in a jar containing a shallow layer of solvent and sealed. As the solvent rises through the paper, it meets the sample mixture which starts to travel up the paper with the solvent.
- The stationary phase is very uniform absorbent paper.
- The mobile phase is a suitable liquid solvent of mixture of solvents.
- It is inexpensive and provides graphic, clear results.
- Is a rapid and reproducible procedure - as long as all conditions are controlled and maintained.
- Accurate for the separation and identification of many important organic and inorganic compounds.
Thin Layer Chromatography (TLC):
- It involves a stationary phase of a thin layer of adsorbent like silica gel, alumina, or cellulose.
- Advantage of faster runs, better separations, and the choice between different adsorbents
- Is a procedure of chemistry that can identify over 700 types of biochemicals, including significant medical drugs of legal importance.
- Reliable, rapid and easy procedure.
- Relatively inexpensive.
- Able to detect upward of 700 different types of drugs and medications.
By: Hikari Hoshika
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